Telomere length profiles in primary human peritoneal mesothelial cells are consistent with senescence

摘要

Mesothelial cell (MC) senescence contributes to malignancy and tissue fibrosis. The\udrole of telomere erosion in MC senescence remains controversial, with evidence for\udboth telomere-dependent and telomere-independent mechanisms reported. Single\udtelomere length analysis revealed considerable telomere length heterogeneity in\udfreshly isolated human peritoneal MCs, reflecting a heterogeneous proliferative history\udand providing high-resolution evidence for telomere-dependent senescence. By\udcontrast the attenuated replicative lifespan, lack of telomere erosion and induction of\udp16 expression in in vitro-aged cells was consistent with stress-induced senescence.\udGiven the potential pathophysiological impact of senescence in mesothelial tissues,\udhigh-resolution MC telomere length analysis may provide clinically useful information.